Preparation of Ξ²-trypsin by affinity chr
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Juris J. Liepnieks; Albert Light
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Article
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1974
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Elsevier Science
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English
β 609 KB
The preparation of /3-trypsin can be simply accomplished from the activation of bovine trypsinogen with partially purified enterokinase in the presence of STI-Sepharose. Enterokinase catalyzes the specific cleavage of lysine 6-isoleucine 7 and the presence of ST1 prevents autolysis of /3-trypsin by