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The kinetics of trypsinogen activation studied by analytical affinity chromatography

✍ Scribed by Volker Kasche


Book ID
115702938
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
357 KB
Volume
173
Category
Article
ISSN
0003-9861

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Preparation of Ξ²-trypsin by affinity chr
✍ Juris J. Liepnieks; Albert Light πŸ“‚ Article πŸ“… 1974 πŸ› Elsevier Science 🌐 English βš– 609 KB

The preparation of /3-trypsin can be simply accomplished from the activation of bovine trypsinogen with partially purified enterokinase in the presence of STI-Sepharose. Enterokinase catalyzes the specific cleavage of lysine 6-isoleucine 7 and the presence of ST1 prevents autolysis of /3-trypsin by