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The kinetics of chick cell population aging in vitro

✍ Scribed by J. M. Ryan

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
701 KB
Volume
99
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

We have examined the kinetics of chick cell population aging in vitro using the percentage of labeled nuclei, the number of colonies formed from a low density inoculum and the number of cells/colony to monitor culture age. The results from these studies showed a gradual age‐associated decline in each of the parameters which was first detected early in the culture lifespan and well in advance of changes in total cell number at confluency. Our results also indicated that each of the above parameters, in addition to the calendar time cells had been in culture, could be used to estimate the percentage of lifespan completed by the culture. A comparison of the methods used to estimate the remaining culture lifespan indicated that the percentage of labeled nuclei was the most accurate in describing cell age.

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