The kinetics of activated uricase immobilized on a polypyrrole film

✍ Scribed by Mu Shaolin

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 368 KB
• Volume: 39
• Category: Article
• ISSN: 0013-4686
• DOI: 10.1016/0013-4686(94)85004-6

✦ Synopsis

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-The effect of 15 metal cations and NH: on the response current of an inactive pofypyrrole uricase electrode has been investigated. Uricase is activated only by MnZ+ among the investigated cations. The response current of the uricase electrode increases with increasing the concentrations of Mn2+ and uric acid. Based on the experimental fact, a mechanism of the activation kinetics of immobilized uricase in the presence of bin' l is presented, that is, E + M = EM, EM + S r= EMS -D EM + P, and a rate equation from this model is given. The activation time of the immobilimd uricase relates to an applied potential and less than 10s at potentials above 0.375 V.