The kinetics and mechanism of shear inactivation of lipase from Candida cylindracea
β Scribed by Yuan-Kun Lee; Choon-Liang Choo
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 761 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Lipase B from Candida antarctica (CAL-B) catalyses the highly enantioselective (E > 200) transesterification of some 1-biaryl-2-yl-, -3-yl-, and -4-ylethanols and -propan-1-ols, as well as 1-(o-, m-, and p-pyridylphenyl)ethanols, 6, with vinyl acetate, Kazlauskas rule being obeyed in all cases. meta
## Abstract The engineering of lipase B from __Candida antarctica__ (CALB) by circular permutation has yielded over sixty hydrolase variants, and several show significantly improved catalytic performance. Here we report a detailed characterization of ten selected enzyme variants by kinetic and spec