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The kinetic mechanism of the hairpin ribozyme in vivo: influence of RNA helix stability on intracellular cleavage kinetics

✍ Scribed by Christine P. Donahue; Ramesh S. Yadava; Steven M. Nesbitt; Martha J. Fedor

Book ID
115629891
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
310 KB
Volume
295
Category
Article
ISSN
0022-2836

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## Abstract Small interfering RNA (siRNA) molecules achieve sequence‐specific gene silencing through the RNA interference (RNAi) mechanism. Here, live‐cell and live‐animal bioluminescent imaging (BLI) is used to directly compare luciferase knockdown by unmodified and nuclease‐stabilized siRNAs in r