The isolation of mutants not accumulating poly-?-hydroxybutyric acid

✍ Scribed by Schlegel, H. G. ;Lafferty, R. ;Krauss, I.

• Book ID: 104762481
• Publisher: Springer-Verlag
• Year: 1970
• Weight: 676 KB
• Volume: 71
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00410161

✦ Synopsis

Five mutant strains of Hydrogenomonas H 16 which synthesize poly-fi-hydroxybutyric acid either slowly or not at all have been isolated following nitrite and NMG treatment of wild type cells. When grown on a nitrogen deficient agar medium, the colonies of PHB-free cells can be recognized by their diminished retention of the dye sudanblack B. Enrichment procedures for such mutants have been devised employing the ~2P-phosphate inactivation technique and sucrose gradient centrifugation. The mutants have been characterized with respect to their growth properties, respiratory control and other properties.