The isolation of microsomal subfractions of mouse plasmacytoma cells: The effect of salt concentration during nitrogen cavitation

✍ Scribed by Asbjörn M. Svardal; Ian F. Pryme

• Book ID: 102629970
• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 311 KB
• Volume: 89
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90359-7

✦ Synopsis

Following disruption of MPC-11 cells by nitrogen cavitation the microsomes have been fractionated by centrifugation on discontinuous sucrose gradients. When the homogenization buffer contained 25 mM KC1 three fractions were observed: smooth microsomes, light rough microsomes, and heavy rough microsomes. When it contained 108 mM KCl, however, only smooth and light microsomal fractions were found. Under the latter conditions the heavy rough microsomal vesicles were apparently not released as separate organelles but instead sedimented together with the endoplasmic reticulum which remains attached to the nuclei.