A procedure to isolate high-molecular-weight DNA from plant materials has been devised. With this procedure, high-molecular-weight DNA suitable for Southern transfer experiments has been isolated from over 30 plant species including angiosperms (both dicots and monocots), a gymnosperm, members of ot
The isolation of high molecular weight DNA from whole organisms or large tissue masses
β Scribed by Dale E. Graham
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 303 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
The isolation of high molecular weight DNA from whole organisms (or large tissue masses) is improved by the disruption of tissues by freeze-grinding and the rapid introduction of organic deproteinizers.
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