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The Isolation of Complementary Strands from a Mouse DNA Fraction

โœ Scribed by W. G. Flamm, Mora McCallum and P. M. B. Walker


Book ID
123647883
Publisher
National Academy of Sciences
Year
1967
Tongue
English
Weight
696 KB
Volume
57
Category
Article
ISSN
0027-8424

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A general method has been developed for the large scale isolation of intact, circular, single-stranded DNA molecules of each strand from supercoiled duplex DNA. The method involves the conversion of the supercoiled duplex DNA to singly nicked, relaxed duplex DNA; denaturation of the duplex DNA; sepa