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The isolation of cDNA clones for CD48

✍ Scribed by Hilary A. Vaughan; Margaret M. Henning; Damian F. J. Purcell; Ian F. C. McKenzie; Mauro S. Sandrin

Publisher: Springer-Verlag
Year: 1991
Tongue: English
Weight: 402 KB
Volume: 33
Category: Article
ISSN: 0093-7711
DOI: 10.1007/bf00210824

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✦ Synopsis

HuLy-m3 is an Mr 47,000 pan-leukocyte antigen detected by the monoclonal antibody (mAb) 5-4.8. This report describes the isolation and analysis of a cDNA clone encoding HuLy-m3. Serological analysis demonstrated that antibodies of the CD48 cluster also reacted with transfected cells expressing HuLy-m3. The DNA sequence of the clone suggests linkage to the cell membrane through a glycosyl phosphatidylinositol tail and this was verified experimentally. Sequence similarity with the human B-cell activation antigen Blast-1 was noted.

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