The isolation and characterization of missense mutants in the general repressor protein Ssn6 of Saccharomyces cerevisiae
โ Scribed by Limbach, M. P. ;Zitomer, R. S.
- Book ID
- 106133394
- Publisher
- Springer
- Year
- 2000
- Tongue
- English
- Weight
- 163 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The mutant tmpl--10ts which confers thermosensitive auxotrophy for thymidylate is employed for the selection of 5'-dTMP uptaking mutants. At the nonpermissive temperature yeast cells phenotypically wild type for thymidylate uptake can grow for only 3 to 4 generations in the presence of 10(-2) M 5'-d
Sporulation-deficient mutants were isolated from a homothallic strain of Saccharomyces cerevisiae. Sporulation was induced in these mutants by procedures to sporulate the products of protoplast fusion between mutants and wild-type strains. Spores formed in this way were crossed to wild-type strains