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The Isolation and Characterisation of Nerve Growth Factor from the Prostate Gland of the Guinea-Pig

✍ Scribed by Carey A. CHAPMAN; Charles A. VERNON; Barbara E. C. BANKS; John M. WALKER


Book ID
115119929
Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
510 KB
Volume
115
Category
Article
ISSN
1432-1327

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## Abstract Nerve growth factor (NGF) has been purified from the guinea pig prostate using a modification of the Bocchini‐Angeletti method for isolating 2.5S NGF from mouse submaxillary glands. As with the mouse preparation, guinea pig prostate NGF appears to migrate as a high molecular weight enti

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Prostate glands of adult guinea pigs were stained for nerve growth factor (NGF) and epidermal growth factor (EGF) by immunohistochemical methods. Both NGF and EGF were localized diffusely in the cytoplasm of the glandular epithelial cells, and also in their secretory products. These findings suggest

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Guinea pig prostate contains one major soluble esteropeptidase activity. The protein has been purified and characterized and found to be a glycoprotein comprised of a single polypeptide chain. The molecular weight of the deglycosylated protein is approximately 26,000. The esteropeptidase has a simil