𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The involvement of hypoxia-inducible factor-1α in the susceptibility to γ-rays and chemotherapeutic drugs of oral squamous cell carcinoma cells

✍ Scribed by Eri Sasabe; Xuan Zhou; Dechao Li; Naohisa Oku; Tetsuya Yamamoto; Tokio Osaki

Publisher
John Wiley and Sons
Year
2006
Tongue
French
Weight
898 KB
Volume
120
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The transcription factor hypoxia‐inducible factor‐1α (HIF‐1α) is the key regulator that controls the hypoxic response of mammalian cells. The overexpression of HIF‐1α has been demonstrated in many human tumors. However, the role of HIF‐1α in the therapeutic efficacy of chemotherapy and radiotherapy in cancer cells is poorly understood. In this study, we investigated the influence of HIF‐1α expression on the susceptibility of oral squamous cell carcinoma (OSCC) cells to chemotherapeutic drugs (cis‐diamminedichloroplatinum and 5‐fluorouracil) and γ‐rays. Treatment with chemotherapeutic drugs and γ‐rays enhanced the expression and nuclear translocation of HIF‐1α, and the susceptibility of OSCC cells to the drugs and γ‐rays was negatively correlated with the expression level of HIF‐1α protein. The overexpression of HIF‐1α induced OSCC cells to become more resistant to the anticancer agents, and down‐regulation of HIF‐1α expression by small interfering RNA enhanced the susceptibility of OSCC cells to them. In the HIF‐1α‐knockdown OSCC cells, the expression of P‐glycoprotein, heme oxygenase‐1, manganese‐superoxide dismutase and ceruloplasmin were downregulated and the intracellular levels of chemotherapeutic drugs and reactive oxygen species were sustained at higher levels after the treatment with the anticancer agents. These results suggest that enhanced HIF‐1α expression is related to the resistance of tumor cells to chemo‐ and radio‐therapy and that HIF‐1α is an effective therapeutic target for cancer treatment. © 2006 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Thrombin induces expression of twist and
Thrombin induces expression of twist and cell motility via the hypoxia-inducible factor-1α translational pathway in colorectal cancer cells
✍ Li-Hsun Chang; Chun-Han Chen; Der-Yi Huang; Hui-Chen Pai; Shiow-Lin Pan; Che-Min 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 407 KB

## Abstract Deep vein thrombosis associated with advanced cancer is known as Trousseau's syndrome. We hypothesized that thrombin, an activator of protease‐activated receptor (PAR)‐1 and PAR‐4 contributes to tumor metastasis. In this study, we demonstrated that thrombin and the PAR‐1 activating pept

Mn-SOD antisense upregulates in vivo apo
Mn-SOD antisense upregulates in vivo apoptosis of squamous cell carcinoma cells by anticancer drugs and γ-rays regulating expression of the BCL-2 family proteins, COX-2 and p21
✍ Eisaku Ueta; Kazunori Yoneda; Tsuyoshi Kimura; Yukihiro Tatemoto; Sayoko Doi; Te 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 388 KB 👁 2 views

ROIs and their scavengers are associated with apoptosis induction by anticancer drugs and ␥-rays, but the details have not been clarified. We examined the effect of transfection of Mn-SOD antisense on apoptosis by 5-FU, PLM, CDDP and ␥-rays using nu/nu mice. After inoculation of Mn-SOD antisense-tra

Antiangiogenic activity of genistein in
Antiangiogenic activity of genistein in pancreatic carcinoma cells is mediated by the inhibition of hypoxia-inducible factor-1 and the down-regulation of VEGF gene expression
✍ Peter Büchler; Howard A. Reber; Markus W. Büchler; Helmut Friess; Robert S. Lave 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 281 KB 👁 2 views

## Abstract ## BACKGROUND Previous reports indicate that Genistein, a naturally occurring isoflavonoid, exhibits strong antiangiogenic activity. The underlying mechanism of inhibition, however, remains unclear. Among the biologic effects of Genistein are the inhibition of tyrosine kinases and the

IL-12- and IL-2-induced tumor regression
IL-12- and IL-2-induced tumor regression in a new murine model of oral squamous-cell carcinoma is promoted by expression of the CD80 co-stimulatory molecule and interferon-γ
✍ Giovana R. Thomas; Zhong Chen; Ileana Enamorado; Caren Bancroft; Carter Van Waes 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 313 KB 👁 2 views

Therapy with IL-12 or IL-2 induces tumor regression in only a few patients with head-and-neck squamous cell carcinoma (SCC), and the factors promoting responsiveness have not been well defined. In this study, we examined whether combined IL-12 and IL-2 therapy can induce tumor regression in a new mu