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The involvement of cyclic GMP in tyrosine aminotransferase degradation in rat hepatoma tissue culture cells

✍ Scribed by Sarah Taylor Strindent; Robert H. Stellwagen

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
583 KB
Volume
108
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Theophylline, a cyclic nucleotide phosphodiesterase inhibitor, increases the rate of tyrosine aminotransferase (TAT) degradation in rat hepatoma tissue culture (HTC) cells. Theophylline (0.1–10 mM) causes a two‐ to five‐fold increase in intracellular cAMP concentration but a 30–60% decrease in cGMP concentration. The decrease in cGMP occurs at doses of theophylline which increase the rate of TAT degradation. When cGMP levels are increased by incubating the cells with either Mn^2+^, an activator of guanylate cyclase, or 8‐bromo‐cGMP, an analog of cGMP, the effect of theophylline is reversed and the rate of TAT degradation is slowed. Thus, the rate of TAT degradation is inversely related to the concentration of cGMP in HTC cells. This raises the possibility that a cGMP‐dependent event is involved in the control of specific protein degradation.

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