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The interaction of auramine o with some synthetic polycarboxylic acids

✍ Scribed by Christian Braud

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
395 KB
Volume
13
Category
Article
ISSN
0014-3057

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✦ Synopsis

The interaction of Auramine O (AuO), a cationic dye, with copolymers of acrylic acid with N-(sec-butyl)-N-methyl-acrylamide, poly(acrylic acid) and poly(methacrylic acid) has been studied during neutralization using spectrofluorimetric and absorption spectroscopic data for the dye. A very large polymer-to-dye ratio (200) has been used to avoid any significant influence of the dye on the conformational behaviour of the polyacids. It has been shown that the binding of AuO is mainly governed by electrostatic attraction from --COO . The enhancement of the fluorescence of AuO in the presence of polyacids with hydrophobic character is attributed to decrease of the freedom of the dye due (it to its insertion into the polyion compact conformations (this appears as the main factor) and (ii) to the presence of apolar groups in the neighbourhood of the dye for open solvated polyions.

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