The interaction of 2,4-dinitrophenol with phospholipids at phospholipid-water interfaces

✍ Scribed by G.T. Rich

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 587 KB
• Volume: 10
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(73)90005-4

✦ Synopsis

The effect of 2, 4-dinitrophenol, DNP, on monolayers of egg lecithin, hydrogenated egg lecithin, dipalmitoyl lecithin and mitochondrial lipids has been examined. Both the undissociated and dissociated forms of DNP bind to the phospholipid polar groups. Binding of the acid form leads to a decrease in monolayer surface potential and an expansion of the monolayer. The amount of penetration of the acid form into lecithin monolayers appears to depend on the London-Van der Waals attractions between the lecithin hydrocarbon chains. Binding of the 2,4-dinitrophenolate anion is reflected in a decrease in surface potential for lecithin monolayers, and an increase in surface potential for mitochondrial lipid monolayers. The adsorption of dinitrophenolate to egg lecithin has been further investigated by micro-electrophoresis of lecithin liposomes. It ls suggested that binding of DNP to phospholipid-water interfaces is important in determining its action as an uncoupler of oxidative phosphorylation, and as a compound that increases the electrical conductance of artificial lipid membranes.

G.T. Rich, 2, 4-Dbfftrophenol at phospholipid-water interfaces