The intensification of absorbance changes in leaves by light-dispersion
✍ Scribed by W. Rühle; A. Wild
- Publisher
- Springer-Verlag
- Year
- 1979
- Tongue
- English
- Weight
- 566 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
In dispersive samples, like leaves, the absorbance of pigments is intensified. The intensification is due to a longer optical path through the dispersive sample. However, in chloroplast suspensions the optical path is not much longer than in clear solutions. The factor of intensification fi (= the lengthening of the optical path) is calculated by comparing the absorbance of leaves and the absorbance of chloroplast suspensions with equal pigment-content. This method also includes the influence of possible sieve effects which could decrease absorbance. The measurements are carried out with high-and low-light leaves of different thickness and pigment content. The intensification of absorbance was 2 2.5 fold. In highlight leaves it was somewhat less than in low-light leaves. The factor fi is better correlated to the pigment content than to the thickness of the leaves. The plot of absorbance versus the pigment content of the leaves shows that fi decreases with increasing pigment content. In contrast, chloroplast suspensions show a linear dependence as expected from Lambert-Beer's law. Thus, in leaves with very low pigment content the absorbance is intensified up to 6 fold while the intensification decreases with increasing absorbance. These results are in good agreement with measurements of Tsel'niker (1975) and with the theoretical predictions of Butler's formula (1960). Absorbance changes due to photooxidation of P-700 and cytochrome f in intact leaves are measured, and fi is used to calculate the amount of the oxidized components. Without correction for p the values would be much greater than the amount actually present. The corrected data show that between 70 and 90% of Abbreviations." A-absorbance; fl-factor of intensification= lengthening of the optical path; Chl=chlorophyll a+b content; DCMU = 3-(3,4-dichlorophenyl)-1,1-dimethylurea; FW-fresh weight; HL=high-light; LA~leaf area; LL=low-light; PhAR photosynthetically active radiation the present P-700 and cytochrome f can be photooxidized in the intact leaf.
📜 SIMILAR VOLUMES
P700 absorption change signals were measured at 800 nm for plant leaves and plant leaf extracts by illuminating with far-red light. The ratio of the signal amplitudes for the two sample types shows the degree of optical signal intensification. The intensification is due to optical path-length elonga