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The influence of temperate bacteriophage φ105 on transformation and transfection in Bacillussubtilis

✍ Scribed by R.E. Yasbin; F.E. Young

Book ID
118853448
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
315 KB
Volume
47
Category
Article
ISSN
0006-291X

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Replicating phage DNA extracted from Bacillus subtilis infected with phage phi 105 has a higher activity in transfection than mature DNA. By heteroduplex analysis it was shown that this DNA contains concatemeric molecules. Concatemers, constructed in vitro by treatment of mature DNA with T4-ligase a