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The influence of organic solvents on transphosphatidylation by phospholipase D from peanut in anhydrous organic solvents

✍ Scribed by Cao Dong; Shi Su-jia

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
240 KB
Volume
110
Category
Article
ISSN
1438-7697

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✦ Synopsis

Abstract

Phospholipase D (PLD) is widely used for the transphosphatidylation of phospholipids, which is conventionally performed in biphasic systems. The influence of organic solvents on transphosphatidylation by peanut PLD in anhydrous organic solvents was studied and, for the first time, compared to that of a biphasic system in this paper. The results demonstrated that PLD activity from peanut was influenced by solvents of different polarity in anhydrous organic solvents, and the influence tendency of organic solvents (diethyl ether, chloroform, methylenechloride) on transphosphatidylation by peanut PLD in anhydrous organic solvents was the same as that in a biphasic system consisting of water and a hydrophobic organic solvent.

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