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The influence of lysis buffer composition on the expression and activity of protein tyrosine phosphatase

✍ Scribed by Jennifer Calvert-Evers; Kate Hammond

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
93 KB
Volume
21
Category
Article
ISSN
0173-0835

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✦ Synopsis

The influence of lysis buffer composition on the expression and activity of protein tyrosine phosphatase Lysis conditions are crucial in the extraction and solubilization of phosphotyrosinecontaining proteins in a form that is immunoreactive, undegraded and enzymatically active. To establish optimal experimental conditions, we evaluated protein tyrosine phosphatase enzyme activity and the detection of PTP-1B protein in human acute promyelocytic leukaemic cells, both before and after retinoic acid treatment. We found that the composition of the lysing buffer greatly influenced the efficiency of solubilization, resulting in major alterations in the activity of protein tyrosine phosphatases and on the mobility of PTP-1B protein.

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