The influence of hypotonic treatment on the morphology of meiotic stages I. Prophase of the first meiotic division of male mice, man andLocusta migratoria

✍ Scribed by A. J. J. Dietrich

• Book ID: 104630512
• Publisher: Springer Netherlands
• Year: 1986
• Tongue: English
• Weight: 658 KB
• Volume: 69
• Category: Article
• ISSN: 0016-6707
• DOI: 10.1007/bf00122930

✦ Synopsis

Male mice were treated with hydroxyurea (HU) to obtain a restricted germ cell population and a sequential analysis of the first meiotic prophase was performed. The results from HU-treated mice .were compared with those from untreated mice, human and grasshopper material with and without hypotonic treatment.

In hypotonically treated material the three subclasses of diffuse stages were obvious in all species investigated. In material not treated hypotonically the different classes of prophase stages showed a more gradual course, there was a tendency towards less spreading of the bivalents and no pronounced diffuse substages could be found. The first desynaptic stage in this material could be recognized in most cases as wellspread classical 'pachytene' like bivalents with a pronounced double-threaded structure. The more advanced desynaptic stage in untreated material resembled the diffuse stage as found in plant meiosis.