The kinetics of production of transducing particles for different bacterial markers were followed by premature lysis of Salmonella typhimurium cells infected with P22 phages. The were compared for cells infected with wild type phage or with HT-mutants which show increased transduction frequencies. M
The influence of host DNA replication on the formation of infectious and transducing Mu-particles
โ Scribed by Johanna Teifel; Horst Schmieger
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 377 KB
- Volume
- 184
- Category
- Article
- ISSN
- 1617-4615
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โฆ Synopsis
We have investigated the influence of bacterial DNA replication on the formation of infectious and transducing Mu-particles. The data obtained agree with the previous findings that growth of phage Mu is independent of the host dnaA gene product (Toussaint and Faelen 1974), but required bacterial replication forks (Fitts and Taylor 1980). The replication of transducing DNA during phage development (Teifel and Schmieger 1979) is controlled by the host and is not a precondition for its packaging. Packaging of transducing DNA does not require a a nearby Mu genome.
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