The influence of fatty acid composition on the restoration of succinate-cytochrome c reductase activity by phospholipids in extracted mitochondria

✍ Scribed by G.G. De Pury; F.D. Collins

• Publisher: Elsevier Science
• Year: 1966
• Tongue: English
• Weight: 572 KB
• Volume: 1
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(66)90004-1

✦ Synopsis

The requirement of the electron transport chain for phospholipids, previously shown for beef heart mitochondria, has been confirmed for rat liver mitochondria. The succinatecytochrome c reductase activity of mitochondria whose lipids had been extracted with acetone has been restored by incubating with micelles of phospholipids.

2. Phospholipids that differ only in their fatty acid composition have been prepared from the livers of normal rats and rats deficient in essential fatty acids.

3. Restoration of enzyme activity was, within limits, proportional to the amount of phospholipid bound by mitochondrial protein and, once bound, pbospholipids from normal and deficient rats were equally effective. However, the rate of binding could be influenced by the fatty acid composition of the phospholipids.

4. It is suggested that enzymes of the electron transport chain do not have any special requirement for phospbotipids that contain essential fatty acids.

* The abbreviation MTAA is used for mitochondria which have been treated with aqueous acetone.