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The influence of different isolation procedures and the use of target cells from melanoma cell lines and short-term cultures on the non-specific cytotoxic effects of lymphocytes from healthy donors

✍ Scribed by Jan E. De Vries; Mary Meyering; Anneke Van Dongen; Philip Rümke

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
676 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

In parallel studies the effects of FHL^1^ and PNL on plated melanoma cells from cell lines and short‐term cultures were compared. FHL showed more frequent and also stronger cytotoxic and/or growth‐inhibiting effects than PNL. On melanoma target cells from cell lines both FHL and PNL showed more frequent and stronger cytotoxic and/or growth‐inhibiting effects than on melanoma target cells from short‐term cultures. In the individual donors the percentage of monocytes and EAC‐rosette‐forming cells in FHL was significantly higher than in PNL. A significant correlation was found between multiplication of the melanoma target cells during the test period and an increased susceptibility towards lymphocytes from healthy donors. Melanoma target cells from cell lines were not more fragile, or more susceptible to unfavourable culture conditions than cells from short‐term cultures, since non‐lymphocytic „effector”︁ cells showed much weaker cytotoxic and/or growth‐inhibiting effects than lymphocytes from healthy donors. Cytotoxic effects of lymphocytes from healthy donors were also registered on target cells from a mammary carcinoma and an osteosarcoma cell line. No significant differences in the cytotoxic effects of lymphocytes from healthy donors were observed when tested on mycoplasma‐contaminated melanoma cells and the same cells made mycoplasma‐free. Mitomycin‐C‐treated lymphocytes retained their cytotoxic effects. Lymphocytes from a healthy donor tested on different occasions on the same melanoma cells from a short term culture showed an incidental cytotoxic reaction.

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