The influence of diet on the ability of rat-liver slices to destroy the carcinogen N,N-dimethyl-P-aminoazobenzene
✍ Scribed by C. J. Kensler
- Publisher
- John Wiley and Sons
- Year
- 1948
- Tongue
- English
- Weight
- 404 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0008-543X
No coin nor oath required. For personal study only.
✦ Synopsis
is an important factor in the production of liver tumors in rats when they are fed the carcinogen, N,N-dimethyl-p-aminoazobenzene (DMB) . Several nutritional factors have been shown to influence tumor production by this agent, i.e., riboflavin, biotin,2, l5 protein: and However, whether the influence of the dietary constituents is to be attributed to alterations in the rate or pathway of metabolism of DMB, to a change in the sensitivity of or ability to resynthesize one or more components of hepatic cells, or to other factors remains to be determined.
Since our earlier report that liver slices destroy DMB5 in vitro, the effect of diet on this capacity of liver tissue has been investigated.
Methods
The rats used in these experiments were of the Wistar strain obtained from the Wistar Institute in 1945 and inbred since that time. The animals used for the nutritional experiments weighed between IOO to 150 gm. at the start. The diets used were of the brownrice-carrot type, which have been studied previously by this group for their effect on tumor production by DMB.4
The ability of rat tissue to destroy DMB in vitro was determined in the following manner. The tissues were suspended in 1.9 ml. Ringer phosphate solution, pH 7.4, and 100 pgm. of DMB or other azo dyes added