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The influence of culture medium composition on the incidence of chromosomal breakage

✍ Scribed by Marianne Keck; Ingrid Emerit

Publisher
Springer
Year
1979
Tongue
English
Weight
346 KB
Volume
50
Category
Article
ISSN
0340-6717

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✦ Synopsis

In patients with chromosomal instability and healthy subjects, significant differences were observed in the chromosomal breakage incidence in simultaneous lymphocyte cultures set up with TC medium 199, Eagle's Minimum Essential Medium, or RPMI, or RPMI 1629. The importance of the choice of culture medium for mutagenicity testing and studies of so-called spontaneous breakage is shown. Cultures incubated with TC Medium 199 showed the highest chromosomal breakage incidence.

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