The influence of complement on cytomegalovirus neutralization by antibodies
โ Scribed by H. Kerzel Andersen
- Publisher
- Springer Vienna
- Year
- 1972
- Tongue
- English
- Weight
- 467 KB
- Volume
- 36
- Category
- Article
- ISSN
- 1432-8798
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## Abstract Human cytomegalovirus (CMV) strain Ad 169 was reacted with IgG antibody obtained from infected renal transplant patients, and the degree of neutralization was determined. The mixture of antibody and virus was then incubated with antihuman IgG (AโIgG) or complement (C) to measure additio
## Abstract The influence of human cytomegalovirus (HCMV) strain variation on neutralizing antibody titers was investigated in sequential sera obtained from 12 organ transplant recipients (11 renal transplant recipients, 1 liver transplant patient) suffering from primary or secondary HCMV infection
Two prokaryotically expressed fusion proteins encompassing amino acids 484-650 (AD-I) and 27-100 (AD-2) of glycoprotein gp58/116 of human cytomegalovirus (HCMV) were purified from E. coli lysates and used in ELISA to determine antibody levels in human sera. The specificity of the test was establishe