The influence of calcium pump coupling on the Arrhenius behavior of sarcoplasmic reticulum Ca2+-ATPase
β Scribed by Martin D. King; Peter J. Quinn; Felix M. Munkonge; Thomas D. Madden
- Publisher
- Springer US
- Year
- 1987
- Tongue
- English
- Weight
- 380 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0145-479X
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## Abstract The sarcoplasmic reticulum (SR) was prepared from the ordinary white muscle of carp __Cyprinus carpio__ acclimated to either 10 or 30Β°C over 5 weeks, and its Ca^2+^ βATPase and Ca^2+^ uptake activities measured. The SR Ca^2+^ βATPase activities of the 10 and 30Β°Cβacclimated carp were 0
Coupling of Ca2+ transport to ATP hydrolysis in isolated sarcoplasmic reticulum vesicles has been studied following pulsed additions of either ATP or Ca2+. ATP was infused as a pulse into medium, whose free Ca2+ concentration was maintained constant at saturating levels by a calciumstat procedure, u