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The influence of auxins on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants

✍ Scribed by F. Barro; M. E. Cannell; P. A. Lazzeri; P. Barcelo


Publisher
Springer
Year
1998
Tongue
English
Weight
560 KB
Volume
97
Category
Article
ISSN
0040-5752

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We developed a highly efficient transformation protocol for the PEG-mediated direct transfer of plasmid DNA into protoplasts of haploid Datura innoxia. Vectors harbouring a neomycin phosphotransferase II gene or a hygromycin B phosphotransferase gene under the control of different promoters were use