Transformation of haploidDatura innoxiap
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Thilo Schmidt-Rogge; Martin Meixner; Vibha Srivastava; Sipra Guha-Mukherjee; Ott
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Article
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1993
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Springer
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English
β 880 KB
We developed a highly efficient transformation protocol for the PEG-mediated direct transfer of plasmid DNA into protoplasts of haploid Datura innoxia. Vectors harbouring a neomycin phosphotransferase II gene or a hygromycin B phosphotransferase gene under the control of different promoters were use