## Abstract The helix–coil transition of poly‐L‐lysine (PLL) in water–isopropanol solvent mixtures has been investigated at room temperature by circular dichroism measurements. Within the range of 70%–80% isopropanol concentration (by volume), the polymer undergoes a sharp transition, characterized
The influence of alcohol–water solvents on the conformation of deoxyribonucleic acid
✍ Scribed by E. V. Frisman; A. N. Veselkov; S. V. Slonitsky; L. S. Karavaev; V. I. Vorob'ev
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1974
- Tongue
- English
- Weight
- 555 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
The influence of alcohol–water solvents on the conformation of native DNA was studied by the methods of flow birefrigence and viscometry. Conformational transitions of DNA were observed at low alcohol concentrations corresponding to the destruction of the water spatial structure. A change in the secondary structure of the DNA molecule was observed at high ethanol concentrations and is discussed in the paper.
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## Synopsis $elutions of calf thymus NaRNA in 0.2M NaCl and CsDNA in 0.2M CsCl (fragmented by sonic irradiation to a molecular weighb of about 5 X 106) were examined by electron microscopy, light scattering, and viscosity measurements. Electron microscopy showed that the molecular fragments are ro
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