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The influence of 2-oxoglutarate on the activity of prolyl 4-hydroxylase

✍ Scribed by J.J. Nietfeld; L. De Jong; A. Kemp


Book ID
113263758
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
369 KB
Volume
704
Category
Article
ISSN
0167-4838

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Assay for 2-oxoglutarate decarboxylating
✍ Gunhild Kaule; Volkmar GΓΌnzler πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 739 KB

A method for the assay of 2-oxoglutarate decarboxylating enzymes based on determination of the reaction product [1-14C]succinate after precipitation of remaining 2-oxo[5-14C]glutarate with 2,4-dinitrophenyl hydrazine is reported. It is particularly useful for the study of the 2-oxoglutarate-coupled