The incorporation of sugar moieties to neuropeptides: comparative study of different methods

✍ Scribed by J.L. Torres; I. Haro; E. Bardaji; G. Valencia; J.M. Garcia-Anton; F. Reig

• Book ID: 104204946
• Publisher: Elsevier Science
• Year: 1988
• Tongue: French
• Weight: 393 KB
• Volume: 44
• Category: Article
• ISSN: 0040-4020
• DOI: 10.1016/s0040-4020(01)89802-1

✦ Synopsis

By using both ~-N-glycosylation and ~-O-glycosylation procedures, different methods for the incorporation of glucose moieties to proline, hydroxyproline or glutamic acid containing protected neuropeptides have been examined. As far as glycosylation of Glu and Hyp containing fragments is concerned, the incorporation of either P-~-N-glucosylated glutamic acid or 4-~-0-glucosylated hydroxyproline to the rest of the peptide have been chosen. However, in the case of C-terminal proline containing peptide fragments, direct ~-N-glucosylation of the full peptide has been preferred. Acetyl protecting groups on the sugar moiety led to better yields than the bulkier benzyl groups.