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The in vitro reconstitution of rough endoplasmic reticulum membrane derived from human placenta

โœ Scribed by Alma L. Gal; Rachel Folman; Henryk H. Czosnek; Yoseph Shiklosh; Nathan de Groot; Abraham A. Hochberg

Book ID
118938047
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
516 KB
Volume
21
Category
Article
ISSN
0024-3205

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The isolation of rough and smooth endoplasmic reticulum from rat parotid salivary gland is described. The rough membrane was stripped of its bound ribosomes using the KCl-puromycin method. Rough endoplasmic reticulum was reconstituted from stripped-rough membrane and polyribosomes. The reconstituted