The in Vitro Growth of Human Chondrocytes
β Scribed by P.E. Harrison; I.K. Ashton; W.E.B. Johnson; S.L Turner; J.B. Richardson; B.A Ashton
- Book ID
- 110287213
- Publisher
- Springer
- Year
- 2000
- Tongue
- English
- Weight
- 49 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1389-9333
No coin nor oath required. For personal study only.
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Adult human articular chondrocytes were expanded in a medium with 10% serum (CTR) or further supplemented with different mitogens (i.e., EGF, PDGFbb, FGF-2, TGFb1, or FGF-2/TGFb1). Cells were then induced to redifferentiate in 3D pellets using serum-supplemented medium (SSM), serum-free medium (SFM)
## Abstract Decreasing replicative potential and dedifferentiation of articular chondrocytes during expansion in cell culture are essential limitations for tissue engineering and cell therapy approaches. Telomeres and telomerase play a key role in cell development, aging, and tumorigenesis. There i