✦ LIBER ✦
The importance of the techniques employed during the enzymatic preparation of isolated liver parenchymal cells
✍ Scribed by Douglas R. Labrecque; Nicholas R. Bachur; Janice A. Peterson; Roger B. Howard
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- English
- Weight
- 352 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The endogenous respiration of isolated rat liver parenchymal cells prepared by two enzymatic techniques has been measured. Despite the production of a high percentage of viable cells by both methods, as measured by the exclusion of trypan blue, the cells obtained by one of the techniques consume oxygen at only one‐half the rate of those obtained by the other technique. It is suggested that the lower rate is the result of an insufficient oxygen supply and also a lack of control of the pH of the incubation medium during the preparation of the cells.