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The importance of ornithine as a precursor for proline in mammalian cells

✍ Scribed by Robert J. Smith; James M. Phang

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
483 KB
Volume
98
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Ornithine aminotransferase catalyzes the reversible transamination of L‐ornithine to Ξ΄^1^‐pyrroline‐5‐carboxylate, the immediate precursor of proline. The direction and flux through this pathway in mammalian cells has not been established. Glutamate has generally been considered to be the most important precursor for proline biosynthesis, but recent studies in xiphoid cartilage indicate that a significant fraction of cellular proline is derived from ornithine. Using newly isolated mutant Chinese hamster ovary cells with defined defects in the proline biosynthetic pathways, we now have established that cells can grow at a maximal rate with ornithine as the sole source of proline. Furthermore, we have measured the rate of proline formation from ornithine (1.6 nmol/h/10^6^ cells). Future studies with these mutant Chinese hamster ovary cells may offer insight into the regulatory mechanism which coordinates proline biosynthesis from ornithine and glutamate.

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