The immune response against anti-idiotope antibodies. I. Induction of idiotope-bearing antibodies and analysis of the idiotope repertoire

✍ Scribed by Toshitada Takemori; Hans Tesch; Michael Reth; Klaus Rajewsky

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 787 KB
• Volume: 12
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830121210

✦ Synopsis

Abstract

In the present analysis we dissect the idiotype repertoire, independently of haptenbinding specificity, by immunizing different strains of mice with cross‐linked monoclonal anti‐idiotopet antibodies against antibody B1‐8. B1‐8 is a monoclonal antibody with specificity for the hapten (4‐hydroxy‐3‐nitro‐phenyl)acetyl (NP) and carries a germ line gene‐encoded variable region. The results demonstrate that the expression of B1‐8 idiotopes and their association with eachother and with NP‐binding specificity are strain‐specific. Certain idiotopes are expressed on antibodies differing in antigen‐binding specificity, whereas one of the idiotopes appears strictly associated with NP‐binding antibodies. The genetic analysis provides strong evidence that the strain specificity of the idiotope repertoire is a result of V region polymorphism in the mouse.