The IE2 promoter/enhancer region from mouse CMV provides high levels of therapeutic protein expression in mammalian cells
✍ Scribed by Philippe Chatellard; Renata Pankiewicz; Evelyne Meier; Laurence Durrer; Christophe Sauvage; Markus O. Imhof
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 405 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Abstract
Protein expression in mammalian cells is key for the production and manufacturing of bio‐therapeutics with human‐like properties and activities. As a molecular basis for reaching high protein expression levels, efficient promoter/enhancer systems are a prerequisite. Here we identify a novel enhancer from the mouse cytomegalovirus (CMV) immediate early 2 (IE2) region as a strong expression‐promoting element. We further demonstrate its activity in bi‐directional promoter architecture and apply it to generate production clones for IL‐18BP, a protein with therapeutic indications in autoimmune diseases. These data show that the IE region from mouse CMV, and the IE2 enhancer/promoter in particular, have a broad potential for application in novel gene expression systems for research, development, and manufacturing of protein drugs. Biotechnol. Bioeng. 2007;96: 106–117. © 2006 Wiley Periodicals, Inc.