## Abstract Firefly luciferase (EC 1.13.12.5) (FL) is the key enzyme in the firefly bioluminescence method (FB), which is widely used to determine the viability of living cells. The FB method can also be applied to monitoring the influence of different pollutants, such as pesticides. Firefly lucife
The identity and properties of firefly luciferase inhibitors in urine
β Scribed by Wright W. Nichols; G.D.W. Curtis; H.H. Johnston
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 534 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0003-2697
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In order t o improve calibration o f firefly luciferase signals obtained by injecting the enzyme into single, isolated heart and liver cells w e have investigated w h y the luminescence from cells is greatly depressed compared with in vitro (in mammalian ionic milieu) and w h y the decay o f the int
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