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The identification of εN-(γ-l-glutamyl)-l-lysine cross-links in native wool keratins

✍ Scribed by R.S. Asquith; M.S. Otterburn; J.H. Buchanan; M. Cole; J.C. Fletcher; K.L. Gardner

Book ID
115748706
Publisher
Elsevier Science
Year
1970
Weight
527 KB
Volume
221
Category
Article
ISSN
0005-2795

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Rapid separation and analysis of Nϵ-(γ-g
Rapid separation and analysis of Nϵ-(γ-glutamyl)-L-lysine-and Nϵ-(β-aspartyl)-L-lysine in protein digests
✍ Michael S. Otterburn; William J. Sinclair 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 396 KB

## Abstract The separation and resolution of the isopeptides __N__ϵ(γ‐glutamyl)‐L‐lysine and __N__ϵ(β‐aspartyl)‐L‐lysine formed in heated proteins has been successfully achieved. The method demands a well‐characterised ion‐exchange column and the use of pH 3.40 lithium citrate buffer (0.2 N Li^+^).