The identification of some water-soluble arsenic species in the marine brown algae Fucus distichus
✍ Scribed by Christopher F. Harrington; Abiodun A. Ojo; Vivan W.-M. Lai; Kenneth J. Reimer; William R. Cullen
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 237 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0268-2605
No coin nor oath required. For personal study only.
✦ Synopsis
The extraction and clean-up procedures developed to isolate the water-soluble arsenic species present in the marine macroalga Fucus distichus, from British Columbia, Canada, are described. The arsenic species were extracted into methanol and then subjected to gel-permeation and ion-exchange chromatography. Fractions high in arsenic were identified by using graphite furnace atomic absorption spectroscopy (GF-AAS), and further investigated by using high-performance liquid chromatography coupled to inductively coupled plasma-mass spectrometry (HPLC-ICP MS). By using different HPLC columns and mobile-phase conditions, the four major arsenic-containing compounds present in the macroalga were positively identified as arsenosugars; one minor compound remained unidentified.