The identification of phosphoseryl residues during the determination of amino acid sequence in phosphoproteins

✍ Scribed by W.Douglas Annan; William Manson; John A. Nimmo

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 638 KB
• Volume: 121
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90557-7

✦ Synopsis

A procedure is described whereby phosphorylated seryl residues may be unequivocally identified during the sequential degradation of a polypeptide chain by the Edman technique. The phosphoseryl residue, Ser(P), was first converted by treatment with methylamine in dilute alkali to a /3-methylaminoalanyl residue which was split from the polypeptide by the degradative procedure as the derived phenylthiohydantoin. This was identified by high-performance liquid chromatography. The procedure was highly effective when the Ser(P) occupied an isolated position in a polypeptide chain but was less so when grouped consecutively with other Ser(P).