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The identification and metabolism of GA9and its metabolites in seed coats and shoots of pea, line G2

โœ Scribed by Yu-Xian Zhu; Peter J. Davies


Publisher
Springer Netherlands
Year
1991
Tongue
English
Weight
629 KB
Volume
10
Category
Article
ISSN
0167-6903

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โœฆ Synopsis


H]gibberellin A 9 was applied to shoots or seed parts of G2 pea to produce radiolabeled metabolites. These were used as markers during purification for the recovery of endogenous GA9 and its naturally occurring metabolites. GA9 and its metabolites were purified by HPLC, derivatized and examined by GC-MS . Endogenous GA 9 , GA20 , GA29 and GA 51 were identified in pea shoots and seed coats . GA, -catabolite and GA.-catabolite were also detected in seed coats . GA70 was detected in seed coats following the application of lug of GA9 . Applied ['H]GA9 was metabolized through both the 13-hydroxylation and 2/3hydroxylation pathways. Labeled metabolites were tentatively identified on the basis of co-chromatography on HPLC with endogenous compounds identified by GC-MS . In shoots ['H]GA S, and ['H]GAS , -catabolite were the predominant metabolites after 6 hrs, but by 24 hrs there was little of these metabolites remaining, while ['H]GA29catabolite and an unidentified metabolite predominated . In seed coats ['H]GA 51 was the initial product, later followed by ['H]GA 51 -catabolite and an unidentified metabolite (different from that in shoots), with lesser amounts of ['H]GA20 , ['H]GA 29 and ['H]GA 29 -catabolite . ['H]GA70 was a very minor product in both cases. ['H]GA 9 was not metabolized by pea cotyledons .


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