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The Human Trifunctional Enzyme ofde NovoPurine Biosynthesis: Heterologous Expression, Purification, and Preliminary Characterization

✍ Scribed by Mark T. Poch; Wen Qin; Carol A. Caperelli

Book ID
115645233
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
157 KB
Volume
12
Category
Article
ISSN
1046-5928

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## Abstract We have cloned, over expressed, and purified one of the two catalytic domains (residues Ala^361^ to Gly^468^, ACE‐N) of human somatic angiotensin‐I converting enzyme in __Escherichia coli.__ This construct represents the __N__‐catalytic domain including the two binding motifs and the 23