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The human cytochrome b5gene and two of its pseudogenes are located on chromosomes 18q23, 14q31-32.1 and 20p11.2, respectively

✍ Scribed by Sara J. Giordano; Min Yoo; David C. Ward; Madhvi Bhatt; Joan Overhauser; Alan W. Steggles

Publisher: Springer
Year: 1993
Tongue: English
Weight: 330 KB
Volume: 92
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00420948

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✦ Synopsis

Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b5 gene and two of its pseudogenes (psgb 51 and psgb52 ) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.

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