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The high performance liquid chromatography of enzyme systems relating to purine and pyrimidine metabolism: an overview

✍ Scribed by David Perrett; H. Anne Simmonds

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
559 KB
Volume
4
Category
Article
ISSN
0269-3879

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Enzyme-based high-performance liquid chr
Enzyme-based high-performance liquid chromatography supports as probes of enzyme activity and inhibition: The immobilization of trypsin and Ξ±-chymotrypsin on an immobilized artificial membrane high-performance liquid chromatography support
✍ Wai-Keung Chui; Irving W. Wainer πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 939 KB

Immobilized artificial membrane (IAM) HPLC supports have been used to immobilize the enzymes alpha-chymotrypsin and trypsin. The enzymes were trapped in hydrophobic cavities on the support and were not covalently attached to the IAM surface. The resulting IAM-enzyme supports retained the hydrolytic