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The Hfr status of Pseudomonas aeruginosa is stabilized by integrative suppression

✍ Scribed by Herrmann, Heidrun ;Klopotowski, Tadeus ;Günther, Elisabeth

Book ID
104725475
Publisher
Springer
Year
1986
Tongue
English
Weight
486 KB
Volume
204
Category
Article
ISSN
0026-8925

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✦ Synopsis

A temperature-sensitive mutant (dna-11) with the phenotype of a mutant defective in the initiation of DNA replication, was isolated from an Hfr-like FP2 donor of Pseudomonas aeruginosa. Reversion of its temperature-sensitive character was achieved by integrative suppression rather than by backmutation or an additional suppressor mutation. The dna-11 mutant proved to be helpful in stabilizing the Hfr status of the original host.

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✍ Haas, Dieter ;Watson, John ;Krieg, Rolf ;Leisinger, Thomas 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 656 KB

A derivative of the IncP-1 plasmid RP1, temperature-sensitive for maintenance, was inserted into the Pseudomonas aeruginosa chromosome by selection for a plasmid marker (carbenicillin resistance) at non-permissive temperature. In one strain, PAO 1000, the plasmid was stably integrated in the trpA, B