The hexose transport system in the human K-562 chronic myelogenous leukemia-derived cell

✍ Scribed by J. C. Dozier; D. F. Diedrich; S. J. Turco

• Book ID: 102309327
• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 436 KB
• Volume: 108
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041080110

✦ Synopsis

Abstract

Kinetics of glucose transport in K‐562 cells was studied using 3‐0‐methylglucose, a nonmetabolizable analog of glucose. A K~m~ of 3.7 mM and V~max~ of 32.0 nmoles/minute/10^6^ cells was found for the process. D‐Glucose, phloretin, and phlorizin competitively inhibit the transport of 3‐0‐methylglucose with Ki values of 4.1 mM, 4.1 μM and 225 μM, respectively, whereas L‐glucose did not inhibit transport at all. The results indicate that K‐562 cells, which are known to have erythropoietic characteristics, possess a glucose carrier system similar to the one in adult human erythrocytes. However, the V~max~ data suggest that more copies of the carrier are present in the malignant cell, presumably to support the high rate of anaerobic glycolysis.