The hen's fertile egg screening test (HEST): A comparison between the acute toxicity for chick embryos and rodents of 20 drugs
✍ Scribed by Hideo Nishigori; Masashi Mizumura; Motoharu Iwatsuru
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 579 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0742-2091
No coin nor oath required. For personal study only.
✦ Synopsis
The possibilities of using developing chick embryos for evaluating drug activities and toxicities were studied by determining LDso values for 20 drugs with 14 different pharmacological activities. Fifteen-day old chick embryos received drugs through the air cell and deaths were measured at 48 hr after the treatments. The LDso values were determined and compared to the i.v., i.p., s.c. and p.o. values from mice listed in the Registry of Toxic Effects of Chemical Substance. The systemic toxicity of 15-day-old chick embryos to drugs were similar to those of mice with the following exceptions. The chick embryos seemed to be more sensitive than mice to antineoplastic or antibiotic agents such as actinomycin D and doxorubicin, whereas, LDso values of cholinergic and cholinergic blocking drugs by this method were 10 to 20 fold of LDso (i. v.) of mice. These observations are important for applying the hen's fertile screening test (HEST) to the determination of drug activities other than that of embryo toxicity or teratogenic activity.