The growth of single roots of Artemisia annua in nutrient mist reactors

✍ Scribed by Barbara E. Wyslouzil; Raymond G. Waterbury; Pamela J. Weathers

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 177 KB
• Volume: 70
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/1097-0290(20001020)70:2<143::aid-bit3>3.0.co;2-b

✦ Synopsis

To better characterize the development and growth of hairy roots in a mist-fed root bed, a single root aerosol reactor was developed. Growth kinetics studies were conducted on hairy roots of Artemisia annua as a function of the mist cycle, carrier gas, and nutrient compositions. Sustained rapid growth was only observed when conditioned medium was fed to the roots. The presence of 1% CO(2) in the carrier gas did not enhance the growth kinetics but it did prevent necrosis of the tissue at the highest mist cycle.